KOREF Cell-line distribution
These cell lines were specially deposited at the KCLB and are provided for research purposes only.
Neither the cell lines nor products derived from them may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community.
Any proposed commercial use of these cells, or their products must first be negotiated with the KCLB,
|Cell Line STR
|Origin||B cell; B lymphoblastoid cell line|
|Species||Human - Human|
|Freezing media||RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%|
|Original media||RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, fetal bovine serum (FBS), 10%|
|KCLB media||RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, fetal bovine serum (FBS), 10%|
|Subculture||Collect the cells into 15ml tube and centrifue, aspirate the culture medium, resuspend the pellets, dispense the cells into the culture flask|