KOREF Cell-line distribution

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These cell lines were specially deposited at the KCLB and are provided for research purposes only. Neither the cell lines nor products derived from them may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. Any proposed commercial use of these cells, or their products must first be negotiated with the KCLB,

Items Contents
KCLB No. 60211
Name KOREF
Distributibility Yes
Cell Line STR
Profile
 
Origin B cell; B lymphoblastoid cell line
Species Human - Human
Cellular morphology lymphoblastoid
Growth pattern suspension
Freezing media RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
Original media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, fetal bovine serum (FBS), 10%
KCLB media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, fetal bovine serum (FBS), 10%
Subculture Collect the cells into 15ml tube and centrifue, aspirate the culture medium, resuspend the pellets, dispense the cells into the culture flask

 

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